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isotype control antibody, mouse igg1  (Miltenyi Biotec)


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    Miltenyi Biotec isotype control antibody, mouse igg1
    Isotype Control Antibody, Mouse Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isotype+control+antibody/Isotype+Control+Antibody%2C+mouse+IgG1/custom%40130-113-761%4042727576
    Average 96 stars, based on 207 article reviews
    isotype control antibody, mouse igg1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    FACS:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Single Cell:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Generated:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Centrifugation:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Incubation:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Control:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation
    Article Snippet: GLAST (ACSA-1) Antibody, anti-human/mouse/rat, APC , Miltenyi Biotec, #130–123–555. .. Isotype Control Antibody, mouse IgG2a, APC , Miltenyi Biotec, #130–113–831. .. Animal model , Brain injection coordinates were experimentally tested on an animal cohort and adapted to animal size. Injection precision was visually tested during tissue dissection. Quality of fluid pumping by osmotic minipumps was verified after a priming period and prior to cannula implantation by visual inspection of fluid passed through the catheters. Volume of FC pumped into SN was checked by control of residual fluid in the pump after explantation. Each set of osmotic minipumps had been certified by Alzet pumping rate with standard deviation. Any outlier animals or tissue were excluded. Exclusion criteria for tissue collection were brain haemorrhage, abscess, and weight loss. Only verified tissues were pulled for further processing..

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Article Title: Transcriptional repression by TGIF2 coordinates neurogenic priming and neural stem cell maintenance.
    Article Snippet: The cell suspension was stained with the preabsorbed antibody mCD133– phycoerythrin (PE) at 1:500 dilution [Anti- Mouse- CD133- PE (13A4), eBioscience/Invitrogen, catalog no. 12- 1331- 82]. .. A corresponding isotype control antibody (Mouse IgM- APC, Miltenyi Biotec, catalog no. 130- 093- 176) was added to an isotype control sample in the same dilution. .. Cells were incubated at 4°C in the dark for 25 min, and then 4′,6- diamidino- 2- phenylindole (DAPI) [1:1000 dilution of stock (1 mg/ml); Sigma- Aldrich, catalog no. D9542] was added, followed by another 5 min of incubation.

    Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity
    Article Snippet: To assess IFN-γ secretion of infected cells upon CD96 costimulation, resting CD4 + T cells were infected with HIV-1 NL4-3 WT IRES-eGFP or HIV-1 NL4-3 N − /U − IRES-eGFP and stimulated with anti-CD3/anti-CD28 (1 μg/ml each; clones HIT3a and CD28.2, BioLegend) at 6 hours postinfection. .. Two days postinfection, cells were restimulated with anti-CD3/anti-CD28 (0.5 μg/ml each; clones HIT3a and CD28.2, BioLegend) alone or in combination with anti-CD96 (2.5 μg/ml; clone REA195, Miltenyi Biotec) or with an isotype control antibody (2.5 μg/ml; REA control human IgG1, Miltenyi Biotec). ..

    Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity
    Article Snippet: .. Coating was performed by incubating wells with 70 μl of PBS containing either recombinant human Nectin-1 (5 μg/ml; BioLegend), CD155 (5 μg/ml; BioLegend), anti-CD96 antibody (2.5 μg/ml; clone REA195, Miltenyi Biotec), or an isotype control antibody (2.5 μg/ml; REA control human IgG1, Miltenyi Biotec) for 2 hours at 37°C. ..

    Article Title: Developing SEMA4A-directed CAR T cells to overcome low BCMA antigen density in Multiple Myeloma
    Article Snippet: Isotype Control Antibody, mouse IgG1, APC (clone IS5-21F5) , Miltenyi Biotec , Cat #130-113-196. .. Isotype Control Antibody, mouse IgG2a, APC (clone S43.10) , Miltenyi Biotec , Cat #130-113-269. .. Bacterial and virus strains , , .

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Concentration Assay:

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma.
    Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma
    Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Isotype Control Antibody, mouse IgG2b, PE (Miltenyi Cat. 130-120-698 at manufacturer’s recommended concentration for 15 min, followed by PBS washing, centrifugation (repeat as before) and resuspension. .. The viability dye 7AAD (1:10; Beckman Coulter, A07704) was used to exclude dead cells.

    Staining:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Fluorescence:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Labeling:

    Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation
    Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding isotype control antibody [REA control antibody IgG (Miltenyi, ref. no. 130–104-613)] was added and samples were incubated for 30 min at 37°C, 5% CO 2 in the dark. .. The cells were washed twice with staining buffer and analyzed with a SH800S cell sorter (Sony).

    Clone Assay:

    Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity
    Article Snippet: To assess IFN-γ secretion of infected cells upon CD96 costimulation, resting CD4 + T cells were infected with HIV-1 NL4-3 WT IRES-eGFP or HIV-1 NL4-3 N − /U − IRES-eGFP and stimulated with anti-CD3/anti-CD28 (1 μg/ml each; clones HIT3a and CD28.2, BioLegend) at 6 hours postinfection. .. Two days postinfection, cells were restimulated with anti-CD3/anti-CD28 (0.5 μg/ml each; clones HIT3a and CD28.2, BioLegend) alone or in combination with anti-CD96 (2.5 μg/ml; clone REA195, Miltenyi Biotec) or with an isotype control antibody (2.5 μg/ml; REA control human IgG1, Miltenyi Biotec). ..

    Recombinant:

    Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity
    Article Snippet: .. Coating was performed by incubating wells with 70 μl of PBS containing either recombinant human Nectin-1 (5 μg/ml; BioLegend), CD155 (5 μg/ml; BioLegend), anti-CD96 antibody (2.5 μg/ml; clone REA195, Miltenyi Biotec), or an isotype control antibody (2.5 μg/ml; REA control human IgG1, Miltenyi Biotec) for 2 hours at 37°C. ..



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    96
    Bio X Cell mouse igg2a
    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of <t>immunoglobulin</t> in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and <t>IgG1</t> were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. <t>OVA-IgG1,</t> Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
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    The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biomedical Journal

    Article Title: Anti-PD-L1 improves barrier function and reduces CD103 + DC cell accumulation by regulating gut and lung microbiota and its metabolites to alleviate asthma in juvenile mice

    doi: 10.1016/j.bj.2025.100944

    Figure Lengend Snippet: The effects of PD-L1 blockade on airway hyperresponsiveness, inflammatory cell infiltration, and the expression of immunoglobulin in lung tissues of asthmatic mice were investigated. (A) Hyperresponsive changes in the airways of mice detected after stimulation with different concentrations of acetylcholine. (B) Total cells in BALF. (C) Number of eosinophils in BALF. (D–E) The expression levels of OVA-IgE and IgG1 were measured in BALF. PD-L1, Programmed cell death ligand 1. OVA-IgE, Ovalbumin-specific Immunoglobulin E. OVA-IgG1, Ovalbumin-specific Immunoglobulin G1. Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Based on previous [ , , ] studies with appropriate modifications and our previous pre-experimental protocol, the anti-mouse PD-L1 monoclonal antibody (10 μg/g/mouse, BioXcell, 804722F1B, America) and rat IgG2b (Clone: LTF-2) isotype control antibody (10 μg/g/mouse, BioXcell, 767921D1, America) were administered by i.p. injection on days 0, 7, and 14 of sensitization and 21, 28, and 35 of challenge.

    Techniques: Expressing