isotype control antibody, mouse igg1 (Miltenyi Biotec)
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Isotype Control Antibody, Mouse Igg1, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+control+antibody/Isotype+Control+Antibody%2C+mouse+IgG1/custom%40130-113-761%4042727576
Average 96 stars, based on 207 article reviews
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Flow Cytometry:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or FACS:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Single Cell:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Generated:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Centrifugation:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Incubation:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Control:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: Glial cell-specific proteomic data from the substantia nigra of a rat 6-OHDA and fluorocitrate model of astrocyte death and microglial activation Article Snippet: GLAST (ACSA-1) Antibody, anti-human/mouse/rat, APC , Miltenyi Biotec, #130–123–555. .. Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding Article Title: Transcriptional repression by TGIF2 coordinates neurogenic priming and neural stem cell maintenance. Article Snippet: The cell suspension was stained with the preabsorbed antibody mCD133– phycoerythrin (PE) at 1:500 dilution [Anti- Mouse- CD133- PE (13A4), eBioscience/Invitrogen, catalog no. 12- 1331- 82]. .. A corresponding Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity Article Snippet: To assess IFN-γ secretion of infected cells upon CD96 costimulation, resting CD4 + T cells were infected with HIV-1 NL4-3 WT IRES-eGFP or HIV-1 NL4-3 N − /U − IRES-eGFP and stimulated with anti-CD3/anti-CD28 (1 μg/ml each; clones HIT3a and CD28.2, BioLegend) at 6 hours postinfection. .. Two days postinfection, cells were restimulated with anti-CD3/anti-CD28 (0.5 μg/ml each; clones HIT3a and CD28.2, BioLegend) alone or in combination with anti-CD96 (2.5 μg/ml; clone REA195, Miltenyi Biotec) or with an Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity Article Snippet: .. Coating was performed by incubating wells with 70 μl of PBS containing either recombinant human Nectin-1 (5 μg/ml; BioLegend), CD155 (5 μg/ml; BioLegend), anti-CD96 antibody (2.5 μg/ml; clone REA195, Miltenyi Biotec), or an Article Title: Developing SEMA4A-directed CAR T cells to overcome low BCMA antigen density in Multiple Myeloma Article Snippet: Isotype Control Antibody, mouse IgG1, APC (clone IS5-21F5) , Miltenyi Biotec , Cat #130-113-196. .. Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Concentration Assay:Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma. Article Snippet: .. Flow cytometry analysis and cell sorting Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Article Title: CRISPR screen reveals SOX2 as a critical regulator of CD133 and cellular stress response in glioblastoma Article Snippet: .. Single cell GBM suspensions were generated from adherent cell cultures by dissociating with 3 mL of TrypLE on 15 cm dishes for 5 min at 37 °C followed by collection with PBS and centrifugation at 300 g. Cells were incubated with CD133/2 anti-human, PE-conjugated antibody (Miltenyi Cat. 130-113-186) or Staining:Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding Fluorescence:Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding Labeling:Article Title: Human epidermis models demonstrate mediator role of TLR2 and TLR3 for psoriatic inflammation Article Snippet: .. For staining of TLR2, 1×10 6 cells were resuspended in 100 μL DermaLife K keratinocyte growth medium with 1% FCS, 2 μL of fluorescence labeled antibody [anti-TLR2-PE (Miltenyi, ref. no. 130–127-922)] or the corresponding Clone Assay:Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity Article Snippet: To assess IFN-γ secretion of infected cells upon CD96 costimulation, resting CD4 + T cells were infected with HIV-1 NL4-3 WT IRES-eGFP or HIV-1 NL4-3 N − /U − IRES-eGFP and stimulated with anti-CD3/anti-CD28 (1 μg/ml each; clones HIT3a and CD28.2, BioLegend) at 6 hours postinfection. .. Two days postinfection, cells were restimulated with anti-CD3/anti-CD28 (0.5 μg/ml each; clones HIT3a and CD28.2, BioLegend) alone or in combination with anti-CD96 (2.5 μg/ml; clone REA195, Miltenyi Biotec) or with an Recombinant:Article Title: HIV-1 manipulates CD96 on CD4 + T cells to subvert antiviral immunity Article Snippet: .. Coating was performed by incubating wells with 70 μl of PBS containing either recombinant human Nectin-1 (5 μg/ml; BioLegend), CD155 (5 μg/ml; BioLegend), anti-CD96 antibody (2.5 μg/ml; clone REA195, Miltenyi Biotec), or an |
